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Catalog Number | orb1257176 |
---|---|
Category | Antibodies |
Description | CTCFL Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CTCFL (NP_001255970.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IF: 1:20 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 68kDa |
Target | CTCFL |
UniProt ID | Q8NI51 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | CTCFL, CT27, BORIS, CTCF-T, HMGB1L1, dJ579F20.2 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IF: 1:20 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using CTCFL antibody (orb1257176) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunofluorescence analysis of MCF-7 cells using CTCFL antibody (orb1257176). Blue: DAPI for nuclear staining.
FC, ICC, IHC, IP, SW-Size, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, ICC, IHC, IP, SW-Size, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
ICC, IF, IHC, WB | |
Bovine, Monkey, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ChIP, IF, IH, WB | |
Human, Mouse, Primate, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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