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Catalog Number | orb1519896 |
---|---|
Category | Antibodies |
Description | CTCF Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | BLR041F |
Tested applications | FC, ICC, IHC, IP, SW-Size, WB |
Reactivity | Human, Mouse |
Immunogen | Between 677 and C-terminus |
Antibody Type | Primary Antibody |
Concentration | 100 µg/ml |
Dilution range | WB - 1:1000; IP - 20 ul/1mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | CTCF |
UniProt ID | P49711 |
NCBI | NP_006556.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | CTCFL paralog; MRD21; CCCTC-binding factor (zinc f Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of mouse CTCF by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BL-395-3B3] (orb1519896) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human CTCF by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, Jurkat (10 µg) , Hep-G2, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BL-395-3B3] (orb1519896) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human CTCF by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (orb1519896). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse CTCF by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (orb1519896). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human CTCF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CTCF recombinant monoclonal antibody [BL-395-3B3] (orb1519896) used for IP at 20 µl/mg lysate.
Detection of mouse CTCF by immunocytochemistry. Sample: FFPE section of CT26 cells. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (orb1519896). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human CTCF by immunocytochemistry. Sample: FFPE section of MCF-7 cells. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (orb1519896). Secondary: HRP-conjugated goat anti-rabbit IgG.
FC, ICC, IHC, IP, SW-Size, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
ICC, IHC, IP, SW-Size, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |