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CTCF Antibody (Center)
Catalog Number: orb1926288
| Catalog Number | orb1926288 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This CTCF antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 334-364 amino acids from the Central region of human CTCF. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | P49711 |
| MW | 82785 Da |
| Tested applications | IHC-P, WB |
| Dilution range | WB: 1:2000, WB: 1:2000, IHC-P: 1:25 |
| Clone Number | RB53773 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Transcriptional repressor CTCF, 11-zinc finger pro Read more... |
| Note | For research use only |
All lanes: Anti-CTCF Antibody (Center) at 1:2000 dilution. Lane 1: Jurkat whole cell lysates. Lane 2: MCF-7 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 83 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CTCF Antibody (Center) at 1:2000 dilution. Lane 1: MCF-7 whole cell lysates. Lane 2: Jurkat whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 83 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Staining CTCF in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
