CFC1 Antibody

SKU: orb1247646

Description

CFC1 Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Mouse
• Predicted Reactivity: Human, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 55kDa band in Human Breast, Placenta and Testis lysates after 0.1µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.6kDa according to NP_115934.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Mouse Embryonic Liver shows staining of the hepatocyte cell membranes. Recommended concentration: 1-2µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: HFTGRYCEHDQRRSEC
• Target: CFC1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CFC1, HTX2, CRYPTIC, cripto, FRL-1, cryptic family 1, cripto-1, cryptic, cryptic gene, MGC133213, CR-1, FLJ77897

orb1247646 (2 ug/ml) staining of paraffin embedded Mouse Embryo Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Quick Database Links

Gene Symbol

CFC1

UniProt

P0CG37

RefSeq (Protein):NP_115934.1