CFC1/Cripto-1 Antibody

• Catalog Number: orb19081
• Category: Antibodies
• Description: Goat polyclonal antibody to CFC1
• Target: CFC1 / Cripto-1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Human, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: HFTGRYCEHDQRRSEC
• RRID: AB_10751640
• MW: 24.6
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:32000, WB: 0.1 μg/ml, IHC-P: 1-2 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 55kDa band in Human Breast, Placenta and Testes lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.6kDa according to NP_115934.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti CFC1 antibody, anti HTX2 antibody, anti CRYPT
• Research Area: Cancer
• Note: For research use only
• Entrez: 55997

Immunohistochemical analysis of formalin-fixed and paraffin embedded Mouse Embryo Liver using CFC1 antibody