CFC1 Rabbit Polyclonal Antibody

SKU: orb182563

Description

CFC1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CFC1 (1-100/223aa)
• Target: CFC1
• Molecular Weight: 15 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CFC 1; CFC1; CFC1_HUMAN; CFC1B; CR 1; Cripto 1; Cripto; Cripto FRL 1 cryptic family 1; CRYPTIC; Cryptic family 1; Cryptic family protein 1; Cryptic gene; Cryptic protein; DTGA2; FLJ77897; FRL 1; HTX2; MGC133213.

Paraformaldehyde-fixed, paraffin embedded (Human glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CFC1) Polyclonal Antibody, Unconjugated (orb182563) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Protein: Hela (human) lysate at 40 ug, Primary: rabbit Anti-CFC1 (orb182563) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 15 kD, Observed band size: 15 kD.

Quick Database Links

Gene Symbol

CFC1

UniProt

P0CG37