CREB1 Antibody

SKU: orb1248376

Description

CREB1 Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 45kDa band observed in nuclear lysates of cell lines Jurkat and NIH3T3 (calculated MW of 36.7kDa according to NP_604391.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3µg/mL.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DSQESVDSVTDSQK
• Target: CREB1
• Molecular Weight: Approx 45kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CREB1, cAMP responsive element binding protein 1, CREB, CREB-1, active transcription factor CREB, cAMP-response element-binding protein-1, cAMP-responsive element-binding protein 1, cyclic AMP-responsive element-binding protein 1, transactivator protein

Quick Database Links

Gene Symbol

CREB1

UniProt

P16220

RefSeq (Protein):NP_004370.1, NP_604391.1