Phospho-CREB-1 (Ser133) Rabbit Polyclonal Antibody

• Catalog Number: orb10462
• Category: Antibodies
• Description: Phospho-CREB-1 (Ser133) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human CREB-1 around the phosphorylation site of Ser133 RP(p-S)YR
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 37 kDa
• Target: CREB1
• UniProt ID: P16220
• RRID: AB_10746582
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CREB(phospho-Ser133); p-CREB-1(Ser133); CREB(phosp
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: RSC96 (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA (green).

P-CREB1 was immunoprecipitated from mouse kidney tissue with orb10462 at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution. Lane 1: mouse kidney tissue lysate 10 µg (Input). Lane 2: orb10462 IP in mouse kidney tissue lysate. Lane 3: native rabbit IgG IP in mouse kidney tissue lysate (negative control). Secondary, All lanes: Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebellum (Mouse) Lysate at 40 ug, Lane 3: Kidney (Mouse) Lysate at 40 ug, Lane 4: Huvec (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-CREB-1 (Ser133) (orb10462) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 45 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-CREB-1 (Ser133) Polyclonal Antibody, Unconjugated (orb10462) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Tissue/Cell: orb10462 human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-CREB-1 (Ser133) Polyclonal Antibody, Unconjugated (orb10462) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.