Phospho-CREB(S133) Antibody

SKU: orb1931276

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: WB - 1:500, IHC-P-Leica - 1:500
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD27310
• Immunogen: This CREB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S133 of human CREB.
• Target: CREB1
• Molecular Weight: 35136 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1

Immunohistochemical analysis of paraffin-embedded Human hepatocarcinoma tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Western blot analysis of lysates from Hela cell line, untreated or treated with TPA, 200nM, using Phospho-CREB (S133) Antibody (upper) or tubulin (lower).

Western blot analysis of lysates from Hela cell line, untreated or treated with TPA, 200nM, using Phospho-CREB (S133) Antibody (upper) or tubulin (lower).

Western blot analysis of lysates from Hela cell line, untreated or treated with 20% FBS + 100nM Calyculin A, using Phospho-CREB (S133) Antibody (upper) or tubulin (lower).

Quick Database Links

Gene Symbol

CREB1

UniProt

P16220

RefSeq (Protein):NP_004370.1, NP_604391.1