Collagen Type I, rat Antibody

SKU: orb867290

Description

Rabbit polyclonal antibody to Collagen Type I

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

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Tested Applications	ELISA, IF, IHC-P, RIA, WB
Dilution Range	(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)
Application Notes	Positive Control: Rat skin. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC(P) 60 min at RT or 2-8°C over night

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Immunogen	Purified collagen type I from rat skin
Purification	affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Concentration	app. 1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Quick Database Links

UniProt

P02454

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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RIA

Radioimmunoassay

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