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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | WB: 0.736111111, IHC: 1:100-1:200 |
| Reactivity | Human, Mouse, Rat, Sheep |
| Predicted Reactivity | Aves, Bovine, Canine, Equine, Feline, Gallus, Goat, Guinea pig, Hamster, Primate, Rabbit, Rodent, Xenopus |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide corresponding to amino acid residues specific to the C-terminal region of human collagen I alpha 1 telopeptide, conjugated to keyhole limpet hemocyanin (KLH). |
| Target | Collagen 1, alpha 1 telopeptide |
| Molecular Weight | 140 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS |
| Concentration | Lot Dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Quality Guarantee
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Western blot analysis of rat lung lysate using Collagen I antibody

Immunofluorescence analysis of mouse lung tissue using Collagen I antibody

Immunofluorescence of a transversal section from mouse spinal cord labeling fibers of collagen (Cat. : orb319485, red, 1:200) from the tissue scaffold. The blue is DAPI counterstaining nuclear DNA.

Immunolabeling of a hiPSCs seeded sponge section cultured for 28 days labeling COL1A1 (Cat. orb319485, 1:100, green) expression and nuclei are labeled with DAPI.

Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (Cat. orb319485, 1:100, red) that has formed fibrils in the extracellular matrix. The blue is staining nuclear DNA.

Western blot of rat lung lysate showing specific immunolabeling of the ~140 kDa collagen protein.
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UniProt Details
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Protocol Information
Collagen 1, alpha 1 telopeptide Antibody (orb319485)
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