COL1A1 Rabbit Polyclonal Antibody

SKU: orb331081

Description

Rabbit polyclonal antibody to Collagen I

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Rabbit, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human COL1A1
• Target: COL1A1
• Molecular Weight: 139 kDa
• Purification: Affinity Purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CO1A1_HUMAN antibody, anti-Collagen I antibody, anti-COL1A1 antibody, anti-COL1A2 antibody, anti-Collagen type I alpha 1 antibody, anti-Collagen type I alpha 2 antibody

25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment. An isoform containing the peptide sequence is present at ~31 kDa.

Sample Tissue: Human 293T Whole Cell, Antibody dilution: 3 ug/ml.

Application: IHC, Species+tissue/cell type: Species+tissue/cell type: Mouse blastocyte, Primary antibody dilution: 1:200, Secondary antibody: Anti-rabbit-Alexa 488, Secondary antibody dilution: 1:200.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/ml in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

WB Suggested Anti-COL1A1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:62500, Positive Control: Human Muscle.

Quick Database Links

Gene Symbol

COL1A1

UniProt

P02452

RefSeq (Protein):NP_000079