CLDN1 Antibody

• Catalog Number: orb1239298
• Category: Antibodies
• Description: CLDN1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Bovine, Mouse
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: Anti-CLDN1 antibody (orb1239298) was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of human CLDN1. The immunogen is located within the last 50 amino acids of CLDN1.
• Concentration: 1 mg/mL
• Dilution range: WB: 1-2 μg/mL; ICC: 5 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 23kDObserved: 23 kD
• Target: CLDN1
• UniProt ID: O95832
• NCBI: CAG33419
• Storage: CLDN1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: CLDN1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: CLDN1 Antibody: CLD1, SEMP1, ILVASC, CLD1, UNQ481/
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: CLDN1, orb1239298 (1 µg/mL), CLDN1 (2 µg/mL), and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human HepG2 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: CLDN1, orb1239298 (A: 1 µg/mL and B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Tissue Lysates. Loading: 15 µg of lysates per lane. Antibodies: CLDN1, orb1239298 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Tissue Lysates. Loading: 15 µg of lysates per lane. Antibodies: CLDN1, orb1239298 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of CLDN1 in HepG2 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling CLDN1 with orb1239298 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).