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Item 1 of 4
Item 1 of 4
CLDN1 Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb1172770
| Catalog Number | orb1172770 |
|---|---|
| Category | Antibodies |
| Description | CLDN1 Recombinant Rabbit Monoclonal Antibody |
| Target | CLDN1 |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Purification | Affinity purified by Protein A |
| Immunogen | A synthesized peptide derived from human Claudin 1 (180-211aa) |
| MW | 23 kDa |
| Tested applications | WB |
| Dilution range | WB=1:500-2000 |
| Antibody Type | Primary Antibody |
| Clone Number | B5D10 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | CLD1_HUMAN; Claudin-1; Claudin 1; Claudin1; CLD1; Read more... |
| Research Area | Signal Transduction |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
25 ug total protein per lane of various lysates probed with CLDN1 monoclonal antibody, unconjugated (orb1172770) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Sample: Lane 1: Mouse Skin tissue lysates, Lane 2: Human HepG2 cell lysates, Primary: Anti-CLDN1 (orb1172770) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: kDa, Observed band size: 18 kDa.
Tissue: Human colon cancer, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172770.
Tissue: Human esophagus, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172770.
