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CLDN1 Recombinant Rabbit Monoclonal Antibody

Catalog Number: orb1172770

DispatchUsually dispatched within 5-10 working days
$ 170.00
Catalog Numberorb1172770
CategoryAntibodies
DescriptionCLDN1 Recombinant Rabbit Monoclonal Antibody
TargetCLDN1
ClonalityRecombinant
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Rat
Predicted ReactivityMouse
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human CLDN1
MW23 kDa
Tested applicationsWB
Dilution rangeWB=1:500-2000
Antibody TypeRecombinant Antibody
Clone NumberB5D10
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesCLD1_HUMAN; Claudin-1; Claudin 1; Claudin1; CLD1;
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NoteFor research use only
CLDN1 Recombinant Rabbit Monoclonal Antibody

25 ug total protein per lane of various lysates probed with CLDN1 monoclonal antibody, unconjugated (orb1172770) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

CLDN1 Recombinant Rabbit Monoclonal Antibody

Sample: Lane 1: Mouse Skin tissue lysates, Lane 2: Human HepG2 cell lysates, Primary: Anti-CLDN1 (orb1172770) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: kDa, Observed band size: 18 kDa.

CLDN1 Recombinant Rabbit Monoclonal Antibody

Tissue: Human colon cancer, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172770.

CLDN1 Recombinant Rabbit Monoclonal Antibody

Tissue: Human esophagus, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172770.