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CLDN1 Recombinant Rabbit Monoclonal Antibody

Catalog Number: orb1172770

DispatchUsually dispatched within 5-10 working days
$ 270.00
Catalog Numberorb1172770
CategoryAntibodies
DescriptionCLDN1 Recombinant Rabbit Monoclonal Antibody
Species/HostRabbit
ClonalityRecombinant
Clone NumberB5D10
Tested applicationsWB
Predicted ReactivityMouse
ReactivityHuman, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human CLDN1
Antibody TypeRecombinant Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000
Form/AppearanceLiquid
ConjugationUnconjugated
MW23 kDa
TargetCLDN1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesCLD1_HUMAN; Claudin-1; Claudin 1; Claudin1; CLD1;
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NoteFor research use only
Expiration Date12 months from date of receipt.
CLDN1 Recombinant Rabbit Monoclonal Antibody

25 ug total protein per lane of various lysates probed with CLDN1 monoclonal antibody, unconjugated (orb1172770) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

CLDN1 Recombinant Rabbit Monoclonal Antibody

Sample: Lane 1: Mouse Skin tissue lysates, Lane 2: Human HepG2 cell lysates, Primary: Anti-CLDN1 (orb1172770) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: kDa, Observed band size: 18 kDa.

CLDN1 Recombinant Rabbit Monoclonal Antibody

Tissue: Human colon cancer, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172770.

CLDN1 Recombinant Rabbit Monoclonal Antibody

Tissue: Human esophagus, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172770.