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Catalog Number | orb1294235 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Claudin-1 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide |
Dilution range | Western Blot 1:1000 – 1:2000 Immunofluorescence 1:300 – 1:500 |
Conjugation | Unconjugated |
Target | Claudin-1 / CLDN1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Lysates at 30 μg per lane. This blot was produced using a 15% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294235 overnight at 4°C. Anti-Claudin 1 antibody at 1/1000 dilution.
Immunohistochemical analysis of paraffin embedded Human prostate cancer tissue labeling Claudin-1 with orb1294235 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Claudin-1 with orb1294235 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Claudin-1 with orb1294235 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Claudin-1 with orb1294235 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Claudin-1 with orb1294235 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Claudin-1 with orb1294235 at 1/100.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294235 anti-Claudin 1 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
ICC, IF, IHC | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
The purity information is not available for VLPs proteins. | |
24.1 kDa | |
Mammalian cell |
Greater than 90% as determined by SDS-PAGE. | |
13.4 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
24.2 kDa | |
in vitro E.coli expression system |
WB | |
Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |