CLDN1 Rabbit Polyclonal Antibody

SKU: orb10445

Description

CLDN1 Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse Claudin 1 (121-211/211aa)
• Target: CLDN1
• Molecular Weight: 23 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CLD1_HUMAN; Claudin-1; Claudin 1; Claudin1; CLD1; SEMP1; Senescence-associated epithelial membrane protein; UNQ481/PRO944; ILVASC;

Sample: Lane 1: Mouse Skin tissue lysates, Lane 2: Human A549 cell lysates, Primary: Anti-CLDN1 (orb10445) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 23 kDa, Observed band size: 17 kDa.

Tissue/Cell: rat intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Claudin-1 Polyclonal Antibody, Unconjugated (orb10445) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

CLDN1

Ningbo Qin 1, Hongxu Liu 2, Xinru Wang 2, Yi Liu 2, Hong Chang 2, Xiaodong Xia Sargassum fusiforme polysaccharides protect mice against Citrobacter rodentium infection via intestinal microbiota-driven microRNA-92a-3p-induced Muc2 production Int J Biol Macromol, (2025)

PubMed: 39863236

Applications

WB

Reactivity

Mouse