CLDN1 Rabbit Polyclonal Antibody

• Catalog Number: orb10445
• Category: Antibodies
• Description: CLDN1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse Claudin 1 (121-211/211aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 23 kDa
• Target: CLDN1
• RRID: AB_10928285
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CLD1_HUMAN; Claudin-1; Claudin 1; Claudin1; CLD1;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Ningbo Qin 1, Hongxu Liu 2, Xinru Wang 2, Yi Liu 2, Hong Chang 2, Xiaodong Xia Sargassum fusiforme polysaccharides protect mice against Citrobacter rodentium infection via intestinal microbiota-driven microRNA-92a-3p-induced Muc2 production Int J Biol Macromol, (2025)

PubMed: 39863236

Applications

WB

Reactivity

Mouse

Sample: Lane 1: Mouse Skin tissue lysates, Lane 2: Human A549 cell lysates, Primary: Anti-CLDN1 (orb10445) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 23 kDa, Observed band size: 17 kDa.

Tissue/Cell: rat intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Claudin-1 Polyclonal Antibody, Unconjugated (orb10445) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.