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Catalog Number | orb1256417 |
---|---|
Category | Antibodies |
Description | CHMP4B Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human CHMP4B (NP_789782.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 36kDa |
Target | CHMP4B |
UniProt ID | Q9H444 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Charged multivesicular body protein 4b, Chromatin- Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using CHMP4B antibody (orb1256417) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 20s.
Immunohistochemistry of paraffin-embedded rat lung using CHMP4B antibody (orb1256417) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using CHMP4B antibody (orb1256417) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human rectum using CHMP4B antibody (orb1256417) at dilution of 1:100 (40x lens).
FC, IHC-P, WB | |
Gallus, Mouse, Xenopus, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Mouse, Other, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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