CFTR Antibody

SKU: orb2641998

Description

Cystic fibrosis transmembrane conductance regulator (CFTR) is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (MDR1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CFTR antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: CFTR1-1
• Immunogen: Human recombinant protein from the C-terminal region (within amino acids 1350-1480) was used as the immunogen for this CFTR antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human pancreas with CFTR antibody (clone CFTR1-1). Staining of FFPE tissue is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE mouse kidney with CFTR antibody (clone CFTR1-1). Staining of FFPE tissue is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.

Quick Database Links

UniProt

P13569