MRP1/ABCC1 Rabbit Polyclonal Antibody

SKU: orb11074

Description

MRP1/ABCC1 Rabbit Polyclonal Antibody

Research Area

Cardiovascular Research, Cell Biology, Drug Metabolism, Hormone Biosynthesis, Metabolism

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/test
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Gallus, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MRP1 (501-600/1531aa)
• Target: ABCC1
• Molecular Weight: 170 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Multidrug Resistanec-Associated Protein 1; ABC 29; ABC29; ABCC 1; ABCC; ABCC1; ATP binding cassette sub family C(CFTR/MRP) member 1; ATP binding cassette sub-family C member 1; DKFZp686N04233; DKFZp781G125; GS X; GSX; Leukotriene C(4) transporter; LTC4 transporter; MRP 1; MRP; Multidrug resistance associated protein 1; Multidrug resistance protein; Multiple drug resistance associated protein; Multiple drug resistance protein 1; MRP1_HUMAN.

Blank control: A549. Primary Antibody (green line): Rabbit Anti-ABCC1 antibody (orb11074), Dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: K562. Primary Antibody (green line): Rabbit Anti-MRP1/ABCC1 antibody (orb11074), Dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MRP1 Polyclonal Antibody, Unconjugated (orb11074) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

ABCC1

UniProt

P33527