CDH1 Rabbit Polyclonal Antibody

SKU: orb626599

Description

Rabbit polyclonal antibody to E-cadherin

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: WB: 1:5000-1:20000; IHC: 1:500-1:1000; IF: 1:20-1:100
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: cadherin 1, type 1, E-cadherin(epithelial)
• Target: CDH1
• Molecular Weight: 125 kDa
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: liquid
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using orb626599 (E-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

mouse testis tissue were subjected to SDS PAGE followed by western blot with orb626599 (E-cadherin antibody) at dilution of 1:2000.

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using orb626599 (E-cadherin Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer (pH9).

Quick Database Links

Gene Symbol

CDH1

UniProt

P12830