E cadherin Rabbit Polyclonal Antibody (FITC)

• Catalog Number: orb15536
• Category: Antibodies
• Description: E cadherin Rabbit Polyclonal Antibody (FITC)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human E-cadherin (841-882/882aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=1μg/Test
• Conjugation: FITC
• Target: CDH1
• RRID: AB_10735816
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: E-cadherin; anion exchanger protein 3; Arc 1; Cadh
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Pal, Sumanta K. et al. Detection and phenotyping of circulating tumor cells in high-risk localized prostate cancer Clin Genitourin Cancer, 13, 130-136 (2015)

PubMed: 25450039

Applications

IF

Reactivity

Human

K, Curzytek et al. Immune-Regulatory and Molecular Effects of Antidepressants on the Inflamed Human Keratinocyte HaCaT Cell Line Neurotox Res, (2021)

PubMed: 33945102

Applications

IHC

Reactivity

Human

Blank control: A431. Primary Antibody (green line): Rabbit Anti-E cadherin antibody (orb15536), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-E cadherin antibody (orb15536), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.