CD81 Antibody

SKU: orb1925745

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC - 1:1000
• Reactivity: Human, Rat

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: B3880EV492X15X1
• Immunogen: This CD81 antibody is generated from a mouse immunized with recombinant protein from the human region of human CD81.
• Target: CD81 {ECO:0000303|PubMed:8766544, ECO:0000312|HGNC:HGNC:1701}
• Molecular Weight: 25809 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD81 antigen, 26 kDa cell surface protein TAPA-1, Target of the antiproliferative antibody 1, Tetraspanin-28, Tspan-28, CD81, CD81, TAPA1, TSPAN28

Immunohistochemical analysis of paraffin-embedded Human liver section using Pink1. Diluted at 1:1000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Anti-CD81 Antibody at 1:4000 dilution + human testis whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-CD81 Antibody at 1:4000 dilution + rat cerebellum whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CD81 Antibody at 1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: Ramos whole cell lysate. Lane 3: U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

CD81 {ECO:0000303|PubMed:8766544, ECO:0000312|HGNC:HGNC:1701}

UniProt

P60033