CD81 Rabbit Polyclonal Antibody

SKU: orb1294247

Description

Rabbit polyclonal antibody to CD81

Research Area

Disease Biomarkers

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:500, Immunohistochemistry (Paraffin) 1:100 – 1:200, Flow Cytometry 1:50 – 1:100
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human CD81
• Target: CD81
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Disclaimer: For research use only

Anti-CD81 antibody at 1/500 dilution, Lysates at 40 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294247 overnight at 4°C.

Anti-CD81 antibody at 1/500 dilution, Lysates at 50 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294247 overnight at 4°C.

Anti-CD81 antibody at 1/500 dilution, Lysates at 50 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294247 overnight at 4°C.

Anti-CD81 antibody at 1/500 dilution, Lysates/proteins at 65 µg per lane. This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294247 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294247 anti-CD81 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD81 with orb1294247 at 1/100.

Quick Database Links

Gene Symbol

CD81