CD81 Rabbit Polyclonal Antibody

SKU: orb2099

Description

CD81 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=2ug/Test, ELISA=1:5000-10000
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TAPA1/CD81 (101-210/236aa)
• Target: CD81
• Molecular Weight: 26 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

26 kDa cell surface protein TAPA 1; 26 kDa cell surface protein TAPA-1; 26 kDa cell surface protein TAPA1; CD 81; CD81 antigen; CD81 molecule; CD81_HUMAN; CVID6; S5.7; TAPA 1; Target of the antiproliferative antibody 1; Tetraspanin 28; Tetraspanin-28; Tetraspanin28; Tspan 28; Tspan-28; Tspan28.

Blank control (blue): Jurkat cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD81 antibody (orb2099), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Blank control: Mouse Brain cells (blue). Primary Antibody: Rabbit Anti-CD81 /PE Conjugated antibody, dilution: 5 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/PE (orange), used under the same conditions.

Sample: Lane 1: Lymph node (Mouse) Lysate at 40 ug, Lane 2: Spleen (Mouse) Lysate at 40 ug, Lane 3: Lung (Mouse) Lysate at 40 ug, Lane 4: Thymus (Mouse) Lysate at 40 ug, Lane 5: Raw264.7 (Mouse) Cell Lysate at 30 ug, Lane 6: THP-1 (Human) Cell Lysate at 30 ug, Lane 7: Raji (Human) Cell Lysate at 30 ug, Primary: Anti-CD81 (orb2099) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 26 kD, Observed band size: 25 kD.

Quick Database Links

Gene Symbol

CD81

UniProt

P60033