CD81 Antibody

• Catalog Number: orb1239281
• Category: Antibodies
• Description: CD81 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: CD81 antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human CD81.The immunogen is located within amino acids 30 - 80 of CD81.
• Concentration: 1 mg/mL
• Dilution range: CD81 antibody can be used for detection of CD81 by Western blot at 2.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 26 kDa Observed: 25 kDa
• Target: CD81
• UniProt ID: P60033
• NCBI: NP_004347
• Storage: CD81 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: CD81 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: CD81 Antibody: S5.7, CVID6, TAPA1, TSPAN28, CD81 a
• Note: For research use only
• Application notes: CD81 antibody can be used for detection of CD81 by Western blot at 2.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD81 in human lung tissue lysate with CD81 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking petide.

Immunohistochemistry of CD81 in human lung tissue with CD81 antibody at 5 µg/mL.

Immunofluorescence of CD81 in A549 cells with CD81 antibody at 20 µg/mL.

Immunohistochemistry of CD81 in human brain tissue with CD81 antibody at 2 µg/ml.

Immunofluorescence of CD81 in human brain tissue with CD81 antibody at 10 µg/ml. Green: CD81 Antibody (orb1239281) Blue: DAPI staining.