CD8 antibody

SKU: orb2276518

Description

CD8 antibody validated for immunohistochemistry on 76 different Normal Tissues

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Tonsil (interfollicular areas) Negative Control: Epithelial tissues, majority of cells in germinal centres of lymphatic organs Cellular Localization: Cell surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MSVA-008R
• Immunogen: Synthetic peptide corresponding to CD8α residues within aa135-235 of CD8α
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD8 antigen, alpha polypeptide (p32), T8/Leu-2 T-lymphocyte differentiation antigen, Ly3, LYT3, MAL, T-cell surface glycoprotein CD8 alpha chain

B CLL containing interspersed non neoplastic CD8 positive cells.

Duodenum mucosa Duodenal mucosa containing numerous intraepithelial CD8 positive lymphocytes.

Squamous cell carcinoma containing numerous CD8 positive tumor infiltrating lymphocytes.

Quick Database Links

UniProt

P01732