CD8 antibody

SKU: orb323288

Description

CD8 antibody

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-P, WB
• Dilution range: IHC-P: 1:100-800, WB: 1:100-500, IF/ICC: 1:100-800
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD8. Please contact us for the exact immunogen sequence. The peptide is available as orb374945.
• Target: CD8. Recognizes endogenous levels of CD8 protein
• Molecular Weight: 26 kDa
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Disclaimer: For research use only

IHC-P image of mouse spleen tissue using CD8 antibody (2.5 ug/ml)

WB analysis of rat thymus (lane 1), mouse spleen (lane 2), rat brain (lane 3), human thyroid tumour (lane 4) using CD8 antibody (5 ug/ml)

IHC-P image of mouse spleen tissue using CD8 antibody (2.5 ug/ml)

IHC-P staining of rat thymus tissue using anti-CD8 (2.5 ug/ml)

IF image of rat thymus tissue using anti-CD8 (2.5 ug/ml)

Immunohistochemical staining of mouse lymph node tissue using CD8 antibody (2.5 ug/ml)

Western blot analysis of rat thymus (lane 1), mouse spleen (lane 2), rat brain (lane 3), human thyroid tumour (lane 4) using CD8 antibody (5 ug/ml)

IHC-P image of rat thymus tissue using anti-CD8 (2.5 ug/ml)

IHC-P image of mouse skin tissue using CD8 antibody (2.5 ug/ml)

Immunofluorescence analysis of rat thymus tissue using CD8 antibody (2.5 ug/ml)

Immunohistochemical staining of mouse skin tissue using anti-CD8 (2.5 ug/ml)

Immunohistochemical staining of mouse skin tissue using CD8 antibody (2.5 ug/ml)

Quick Database Links

Gene Symbol

CD8. Recognizes endogenous levels of CD8 protein

UniProt

P07725, P01731, P01732

NCBI Gene ID

925

RefSeq:001139345, 001145873

References

Mahshad Entezam 1, Nader Bagheri 2, Amin Soltani 3, Sayedeh Azimeh Hosseini 4, Pegah Khosravian 5, Gordon A Ferns 6, Mahdi GhatrehSamani 7 Enhanced antitumor immunity in breast cancer: Synergistic effects of ADAM10/ADAM17 inhibition, metabolic modulation, and camptothecin-loaded selenium nanoparticles Int J Pharm, (2024)

PubMed: 39675534

Mohammad Jahan-Mahin 1, Roya Askari 1, Amir Hossein Haghighi 1, Omid Khaiyat The effect of three types of water-based training protocols on thymus atrophy and specific indicators of cellular immune senescence in aged male rats Biogerontology, (2025)

PubMed: 39832052

Applications

IHC

Reactivity

Rat

Urszula Daniluk # 1, Agnieszka Świdnicka-Siergiejko # 2, Jarosław Daniluk 2, Małgorzata Rusak 3, Milena Dąbrowska 3, Katarzyna Guzińska-Ustymowicz 4, Anna Pryczynicz 4, Andrzej Dąbrowski 2 The development of pancreatic cancer is accompanied by significant changes in the immune response in genetically predisposed mice Front Oncol, (2025)

PubMed: 40641916

Applications

IHC

Reactivity

Mouse

Roham Deyhimfar 1, Keykavos Gholami 2, Iman Menbari Oskouie 1, Leila Zareian Baghdadabad 1, Parisa Zahmatkesh 1, Mohammadreza Shoghi 3, Gholamreza Mesbah 4, Fateme Guitynavard 1, Seyed Mohammad Kazem Aghamir 5 High-dose vitamin C improves BCG immunotherapy's efficacy in a murine ectopic model of bladder cancer Pathol Res Pract, (2025)

PubMed: 40907423

Applications

IHC

Reactivity

Mouse

Isocitrate dehydrogenase mutations suppress STAT1 and CD8+ T cell accumulation in gliomas J Clin Invest, 127, 1425-1437 (2017)

PubMed: 28319047

Applications

IF

Reactivity

Mouse