CD8 antibody

SKU: orb10325

Description

CD8 antibody

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: WB: 1:200-800, IHC-P: 1:100-300 (based on 0.5 mg/ml)
• Application Notes:
  This antibody is available without BSA and NaN3, please contact us for details

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD8. Please contact us for the exact immunogen sequence. The peptide is available as orb374713.
• Target: CD8
• Molecular Weight: 26 kDa
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

anti CD 8 antibody, anti CD 8A antibody, anti CD 8B antibody, anti CD8 antibody, anti CD8 antigen alpha polypeptide antibody, anti CD8 antigen, alpha polypeptide (p32) antibody, anti CD8A antibody, anti CD8a antigen antibody, anti CD8a molecule antibody, anti CD8A_HUMAN antibody, anti CD8B antibody, anti CD8b antigen antibody, anti CD8b antigen isoform 2 antibody, anti CD8b molecule antibody, anti CD8B protein antibody, anti CD8B1 antibody, anti Leu2 antibody, anti Leu2 T lymphocyte antigen antibody, anti Ly3 antibody, anti LYT3 antibody, anti MAL antibody, anti MGC119115 antibody, anti OKT8 T Cell antigen antibody, anti p32 antibody, anti T cell antigen Leu2 antibody, anti T cell co receptor antibody, anti T cell surface glycoprotein CD8 alpha chain antibody, anti T cell surface glycoprotein CD8 beta chain antibody, anti T lymphocyte differentiation antigen T8/Leu 2 antibody, anti T-cell surface glycoprotein CD8 alpha chain antibody, anti T-lymphocyte differentiation antigen T8/Leu-2 antibody, anti T8 T cell antigen antibody

Immunohistochemical staining of paraffin embedded rat spleen tissue using CD8 antibody (2.5 ug/ml)

Immunohistochemical staining of human endometrial cancer tissue using anti-CD8 (2.5 ug/ml)

IHC-P image of rat spleen tissue using anti-CD8 (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded human endometrial cancer tissue using anti-CD8 (2.5 ug/ml)

WB analysis of rat thymus (lane 1), rat spleen (lane 2), rat brain (lane 3), human breast cancer (lane 4), human endometrial cancer (lane 5) using CD8 antibody (1 ug/ml)

WB analysis of rat thymus (lane 1) using CD8 antibody (1 ug/ml)

IHC-P image of rat spleen tissue using CD8 antibody (2.5 ug/ml)

Quick Database Links

Gene Symbol

CD8

UniProt

P01732, P05541, P10300

NCBI Gene ID

12525

RefSeq:21, 11, 001081110, 001074579

References

Sagkan Ozturk, Aliye et al. Short term effects of experimental gastric outlet obstruction and truncal vagotomy on gut hormones Biotech Histochem, (2021)

PubMed: 33722110

Applications

IHC

Reactivity

Rat

Chien-Chun Steven Pai et al. Clonal Deletion of Tumor-Specific T Cells by Interferon-? Confers Therapeutic Resistance to Combination Immune Checkpoint Blockade Immunity, 50, 477-492.e8 (2019)

PubMed: 30737146

Applications

IHC-P

Reactivity

Mouse

Chen, Xinran et al. Increased B7-H4 expression during esophageal squamous cell carcinogenesis is associated with IL-6/STAT3 signaling pathway activation in mice Oncol Lett, 13, 2207-2215 (2017)

PubMed: 28454382

Applications

IHC

Reactivity

Mouse

Prashant, Chandravilas Keshvan et al. Fabrication of nanoadjuvant with poly-ε-caprolactone (PCL) for developing a single-shot vaccine providing prolonged immunity Int J Nanomedicine, 9, 937-950 (2014)

PubMed: 24611010

Applications

ELISA

Reactivity

Mouse

Pedro Henrique Felix Silva et al. Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats Mol Oral Microbiol, 38, 158-170 (2023)

PubMed: 36495122

Applications

IHC

Reactivity

Rat

Hekim, Can et al. Dasatinib Changes Immune Cell Profiles Concomitant with Reduced Tumor Growth in Several Murine Solid Tumor Models Cancer Immunol Res, 5, 157-169 (2017)

PubMed: 28073775

Applications

IHC

Reactivity

Mouse

Sadighi, Sanambar et al. Down-regulation of immune checkpoints by doxorubicin and carboplatin-containing neoadjuvant regimens in a murine breast cancer model Iran J Basic Med Sci, 24, 537-544 (2021)

PubMed: 34094037

Applications

IHC

Reactivity

Human

Annen, Ryohei et al. Tumor-Specific Immunoenhancing Effects after Local Cryoablation for Metastatic Bone Tumor in a Mouse Model Int J Mol Sci, 23, 9445 (2022)

PubMed: 36012709

Applications

ELISpot, Immunostaining

Reactivity

Mouse

Ka, Na-Lee et al. IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer Cell Rep, 37, 110138 (2021)

PubMed: 34936865