CD8 antibody

• Catalog Number: orb10325
• Category: Antibodies
• Description: CD8 antibody
• Target: CD8
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Immunogen: KLH conjugated synthetic peptide derived from human CD8. Please contact us for the exact immunogen sequence. The peptide is available as orb374713.
• UniProt ID: P10300, P05541, P01732
• RRID: AB_10749399
• MW: 26 kDa
• Tested applications: ELISA, IHC-P, WB
• Dilution range: WB: 1:200-800, IHC-P: 1:100-300 (based on 0.5 mg/ml)
• Application notes: This antibody is available without BSA and NaN3, please contact us for details
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti CD 8 antibody, anti CD 8A antibody, anti CD 8
• Note: For research use only
• Entrez: 12525
• NCBI: 001081110, 001074579, 11, 21
• Expiration Date: 12 months from date of receipt.

References

Sagkan Ozturk, Aliye et al. Short term effects of experimental gastric outlet obstruction and truncal vagotomy on gut hormones Biotech Histochem, (2021)

PubMed: 33722110

Applications

IHC

Reactivity

Rat

Chien-Chun Steven Pai et al. Clonal Deletion of Tumor-Specific T Cells by Interferon-? Confers Therapeutic Resistance to Combination Immune Checkpoint Blockade Immunity, 50, 477-492.e8 (2019)

PubMed: 30737146

Applications

IHC-P

Reactivity

Mouse

Chen, Xinran et al. Increased B7-H4 expression during esophageal squamous cell carcinogenesis is associated with IL-6/STAT3 signaling pathway activation in mice Oncol Lett, 13, 2207-2215 (2017)

PubMed: 28454382

Applications

IHC

Reactivity

Mouse

Prashant, Chandravilas Keshvan et al. Fabrication of nanoadjuvant with poly-ε-caprolactone (PCL) for developing a single-shot vaccine providing prolonged immunity Int J Nanomedicine, 9, 937-950 (2014)

PubMed: 24611010

Applications

ELISA

Reactivity

Mouse

Pedro Henrique Felix Silva et al. Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats Mol Oral Microbiol, 38, 158-170 (2023)

PubMed: 36495122

Applications

IHC

Reactivity

Rat

Hekim, Can et al. Dasatinib Changes Immune Cell Profiles Concomitant with Reduced Tumor Growth in Several Murine Solid Tumor Models Cancer Immunol Res, 5, 157-169 (2017)

PubMed: 28073775

Applications

IHC

Reactivity

Mouse

Sadighi, Sanambar et al. Down-regulation of immune checkpoints by doxorubicin and carboplatin-containing neoadjuvant regimens in a murine breast cancer model Iran J Basic Med Sci, 24, 537-544 (2021)

PubMed: 34094037

Applications

IHC

Reactivity

Human

Annen, Ryohei et al. Tumor-Specific Immunoenhancing Effects after Local Cryoablation for Metastatic Bone Tumor in a Mouse Model Int J Mol Sci, 23, 9445 (2022)

PubMed: 36012709

Applications

ELISpot, Immunostaining

Reactivity

Mouse

Ka, Na-Lee et al. IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer Cell Rep, 37, 110138 (2021)

PubMed: 34936865

Immunohistochemical staining of paraffin embedded rat spleen tissue using CD8 antibody (2.5 ug/ml)

Immunohistochemical staining of human endometrial cancer tissue using anti-CD8 (2.5 ug/ml)

IHC-P image of rat spleen tissue using anti-CD8 (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded human endometrial cancer tissue using anti-CD8 (2.5 ug/ml)

WB analysis of rat thymus (lane 1), rat spleen (lane 2), rat brain (lane 3), human breast cancer (lane 4), human endometrial cancer (lane 5) using CD8 antibody (1 ug/ml)

WB analysis of rat thymus (lane 1) using CD8 antibody (1 ug/ml)

IHC-P image of rat spleen tissue using CD8 antibody (2.5 ug/ml)