CD45RB Antibody (B-cell marker)

SKU: orb639892

Description

CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG1, kappa
• Clone No.: rPTPRC/1132
• Immunogen: Recombinant full-length human CD45RB protein was used as the immunogen for the recombinant CD45RB antibody.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC staining of FFPE human lymph node with recombinant CD45RB antibody (clone rPTPRC/1132). HIER: steam section in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to staining.

Western blot testing of human spleen lysate with recombinant CD45RB antibody (clone rPTPRC/1132). Expected molecular weight: 147-220 kDa depending on glycosylation level.

SDS-PAGE analysis of purified, BSA-free recombinant CD45RB antibody (clone rPTPRC/1132) as confirmation of integrity and purity.

Quick Database Links

UniProt

P08575