CD35 Antibody

• Catalog Number: orb750194
• Category: Antibodies
• Description: Recognizes a protein of 210-220kDa, which is identified as the Complement receptor 1 or CD35. This mAb is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1/CD35 activity. It is highly specific and shows no cross-reaction with CR2/CD21. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This mAb labels follicular dendritic cells and follicular dendritic cell sarcoma.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Human monocytes were used as the immunogen for this CD35 antibody.
• UniProt ID: P17927
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: SPM554
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Store the CD35 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This CD35 antibody is available for research use only.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with CD35 antibody (SPM554).

SDS-PAGE analysis of purified, BSA-free CD35 antibody (clone SPM554) as confirmation of integrity and purity.