CD3 Rabbit Polyclonal Antibody

SKU: orb1294297

Description

Rabbit polyclonal antibody to CD3

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Dilution Range: Western Blot 1:500 – 1:1000, Immunofluorescence 1:300 – 1:400, Immunohistochemistry (Paraffin) 1:300 – 1:500, Flow Cytometry 1:100 – 1:200
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human CD3
• Target: CD3
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Anti-CD3 antibody at 1/500 dilution, Lysates at 50 µg per lane. This blot was produced using a 12% SDS-PAGE. NC membrane was then blocked for an hour before being incubated with orb1294297 overnight at 4°C.

Cells were fixed with 4% PFA for 10 min, permeabilized with 0.1% NP-40 for 10 min then blocked with 5% BSA for 30 min. orb1294297 anti-CD3 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD3 with orb1294297 at 1/400.

CD3 antibody, orb1294297, Cell: Human peripheral blood, CD3 antibody: 1-100.

Quick Database Links

Gene Symbol

CD3