CD3 Intracellular domain Antibody

• Catalog Number: orb1294242
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CD3 (Intracellular
• Target: CD3 Intracellular domain
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit-IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide / Intracellular domain corresponding to Human/mouse CD3
• Tested applications: IF, IHC, WB
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:300-1:400 Immunohistochemistry (Paraffin) 1:100-1:300
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Immunology & Inflammation
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-CD3 antibody at 1/1000 dilution. Lysates at 40 µg per lane. This blot was produced using a 15% SDS-PAGE. NC membrane was then blocked for an hour before being incubated with orb1294242 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294242 anti-CD3 antibody at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human Hepatocellular cancer tissue labeling CD3 with orb1294242 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Tonsil carcinoma tissue labeling CD3 with orb1294242 at 1/200.

Immunohistochemical analysis of paraffin embedded mouse spleen labeling CD3 with orb1294242 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse spleen labeling CD3 with orb1294242 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse spleen labeling CD3 with orb1294242 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

CD3 antibody, orb1294242, Sample: Human peripheral blood, anti-CD3 antibody at 1:100.