CD3 antibody

• Catalog Number: orb348965
• Category: Antibodies
• Description: CD3 antibody
• Target: CD3E
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Immunogen: KLH conjugated synthetic peptide derived from human CD3. Please contact us for the exact immunogen sequence. The peptide is available as orb374972.
• UniProt ID: P22646, P07766
• MW: 19 kDa
• Tested applications: IHC-P, WB
• Dilution range: IHC-P: 1:100-1000, WB: 1:100-1000, (based on 0.5 mg/ml)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-FLJ18683 antibody, Anti-T3E antibody, Anti-TC
• Note: For research use only
• Entrez: 916
• NCBI: 000733, 000724, 11, 31
• Expiration Date: 12 months from date of receipt.

References

Urszula Daniluk # 1, Agnieszka Świdnicka-Siergiejko # 2, Jarosław Daniluk 2, Małgorzata Rusak 3, Milena Dąbrowska 3, Katarzyna Guzińska-Ustymowicz 4, Anna Pryczynicz 4, Andrzej Dąbrowski The development of pancreatic cancer is accompanied by significant changes in the immune response in genetically predisposed mice Front Oncol, (2025)

PubMed: 40641916

Applications

IHC

Reactivity

Mouse

Immunohistochemical staining of paraffin embedded human thyroid tumour tissue using anti-CD3 (2.5 ug/ml)

Immunohistochemical staining of rat bone marrow tissue using CD3 antibody (2.5 ug/ml)

IHC-P image of rat skin tissue using CD3 antibody (2.5 ug/ml)

IHC-P staining of human thyroid tumour tissue using anti-CD3 (2.5 ug/ml)

Immunohistochemical staining of rat skin tissue using anti-CD3 (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat skin tissue using anti-CD3 (2.5 ug/ml)

Immunohistochemical staining of rat bone marrow tissue using anti-CD3 (2.5 ug/ml)

Immunohistochemical staining of rat spleen tissue using anti-CD3 (2.5 ug/ml)

Western blot analysis of mouse brain (lane 1), rat lung (lane 2), human lung cancer (lane 3), human thyroid tumour (lane 4) using CD3 antibody (1 ug/ml)

IHC-P image of rat thymus tissue using CD3 antibody (2.5 ug/ml)