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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IF, WB |
|---|---|
| Dilution Range | IF - 1:10-50, WB - 1:500-1000, FC - 1:10-50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | B602EV7X6X5 |
| Immunogen | This CD138 Monoclonal antibody is generated from mouses immunized with a KLH conjugated synthetic peptide selected from human CD138. |
| Target | SDC1 (HGNC:10658) |
| Molecular Weight | 32462 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Syndecan-1, SYND1, CD138, SDC1, SDC
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CD138 Antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis
CD138 Antibody western blot analysis in T47D cell line lysates (35 μg/lane). This demonstrates the CD138 antibody detected the CD138 protein (arrow).
Confocal immunofluorescent analysis of CD138 antibody with RPMI8266 cell (above) compared with Jurkat as negative cell line (below). followed by DyLight 488-conjugated goat anti-mouse lgG (H+L) Secondary Antibody (green). DAPI was used to stain the cell nucleus (blue).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002988.3, NP_001006947.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
CD138 Antibody (orb1939238)
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