Syndecan-1 Rabbit Polyclonal Antibody

SKU: orb10289

Description

Syndecan-1 Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cell Biology, Cell Signaling, Glycosaminoglycans (GAGs), Heparan Sulfate ProteoGlycan, Lipid Metabolism, Lipids and Lipoproteins Metabolism, Metabolism, Neurogenesis, Neurology, Neuroscience, Stem Cell, Synapse Markers

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD138 (201-310/310aa)
• Target: SDC1
• Molecular Weight: 60-70 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

SDC1; SDC; SYND1; Syndecan I; SyndecanI; syndecan; Sdc1; Syndecan1; AA408134; AA409076; Sstn; syn-1; CD138; CD 138; CD-138; CD138 antigen; Heparan sulfate proteoglycan fibroblast growth factor receptor; SDC 1; Syndecan1 precursor; SDC1_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (orb10289) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (orb10289) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (orb10289) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Mouse Liver tissue lysates, Lane 3: Human U251 cell lysates, Lane 4: Human A431 cell lysates, Lane 5: Human HepG2 cell lysates, Lane 6: Human HeLa cell lysates, Primary: Anti-Syndecan-1 (orb10289) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kDa, Observed band size: 74 kDa.

Quick Database Links

Gene Symbol

SDC1

UniProt

P18827