Caveolin-3 Antibody

• Catalog Number: orb1247242
• Category: Antibodies
• Description: Caveolin-3 Antibody
• Target: CAV3
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine, Canine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: EHTDLEAQIVKDIH-C
• UniProt ID: P56539
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 23kDa band observed in Human, Mouse and Rat Heart lysates and 20kDa in Pig heart and Skeletal Muscle lysates (calculated MW of 17.3kDa according to NP_203123.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows peripheral staining of fibres. Recommended concentration, 3-5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CAV3, caveolin 3, LGMD1C, LQT9, MGC126100, MGC1261
• Note: For research use only
• NCBI: NP_203123.1

orb1247242 (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247242 (3.8 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247242 (0.1 ug/ml) staining of Pig Skeletal Muscle (A) and Heart (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247242 (0.3 ug/ml) staining of Mouse (A) and Rat (B) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.