CAV3 Antibody

SKU: orb20210

Description

Goat polyclonal antibody to CAV3

Research Area

Cardiovascular Disease, Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:4000, WB: 0.1-0.3 μg/ml, IHC-P: 3-5µg/ml
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  IHC: In paraffin embedded Human Skeletal Muscle shows peripheral staining of fibres. Recommended concentration, 3-5μg/ml.WB: Approx. 23kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 17.3kDa according to NP_203123.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CAV3
• Protein Sequence: EHTDLEAQIVKDIH
• Molecular Weight: 17.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CAV3 antibody, anti caveolin 3 antibody, anti LGMD1C antibody, anti LQT9 antibody, anti MGC126100 antibody, anti MGC126101 antibody, anti MGC126129 antibody, anti VIP-21 antibody, anti VIP21 antibody, anti M-caveolin antibody

Western blot analysis of Human Heart lysate using CAV3 antibody

Immunohistochemical analysis of human skeletal muscle tissue using CAV3 antibody

Western blot analysis of staining of Pig Skeletal Muscle (Lane 1) and Heart (Lane 2) lysates using CAV3 antibody.

Western blot analysis of staining of Mouse (A) and Rat (B) Heart lysates using CAV3 antibody.

0.1 µg/mL staining of Human Skeletal Muscle (A) and Rat Heart (B) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

4 µg/mL staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.

Quick Database Links

Gene Symbol

CAV3

NCBI Gene ID

859