CAV3 Antibody

• Catalog Number: orb20210
• Category: Antibodies
• Description: Goat polyclonal antibody to CAV3
• Target: CAV3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine, Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EHTDLEAQIVKDIH
• RRID: AB_10768332
• MW: 17.3
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:4000, WB: 0.1-0.3 μg/ml, IHC-P: 3-5µg/ml
• Application notes: IHC: In paraffin embedded Human Skeletal Muscle shows peripheral staining of fibres. Recommended concentration, 3-5μg/ml.WB: Approx. 23kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 17.3kDa according to NP_203123.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti CAV3 antibody, anti caveolin 3 antibody, anti
• Research Area: Cardiovascular Disease, Epigenetics
• Note: For research use only
• Entrez: 859

Western blot analysis of Human Heart lysate using CAV3 antibody

Immunohistochemical analysis of human skeletal muscle tissue using CAV3 antibody

Western blot analysis of staining of Pig Skeletal Muscle (Lane 1) and Heart (Lane 2) lysates using CAV3 antibody.

Western blot analysis of staining of Mouse (A) and Rat (B) Heart lysates using CAV3 antibody.

0.1 µg/mL staining of Human Skeletal Muscle (A) and Rat Heart (B) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

4 µg/mL staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.