CAV3 Antibody (N-term)

• Catalog Number: orb1930027
• Category: Antibodies
• Description: CAV3 Antibody (N-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Porcine
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P56539
• MW: 17259 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Research Area: Cancer Biology, Cardiovascular Research, Signal Tr
• Note: For research use only

Western blot analysis of CAV3 antibody (N-term) in 293 cell line lysates (35 ug/lane). CAV3 (arrow) was detected using the purified Pab.

Anti-CAV3 Antibody (N-term) at 1:500 dilution + human normal uterus lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 17 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Flow cytometric analysis of 293 cells using CAV3 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human Skeletal muscle reacted with CAV3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.