Catenin alpha 1 Antibody

• Catalog Number: orb1247702
• Category: Antibodies
• Description: Catenin alpha 1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-KREKQDETQTKIK
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CTNNA1
• UniProt ID: P35221
• NCBI: NP_001894.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: catenin (cadherin-associated protein), alpha 1, 10
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 100kDa band observed in human colon and human skin lysates (calculated MW of 100kDa according to NP_001894.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Prostate and Uterus. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247702 (1 ug/ml) staining of human colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing Human CTNNA1 and probed with orb1247702 (mock transfection in first lane).

orb1247702 (3.75 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247702 (3.75 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.