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| Catalog Number | orb764736 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Catenin-alpha E/N |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Catenin-alpha1. AA range:857-906 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 100 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-CTNNA1 antibody, anti-Catenin alpha-1 antibod Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of Hela cell. 1, Catenin-α E/N Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.
Western Blot analysis of various cells using Catenin-α E/N Polyclonal Antibody diluted at 1:500.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Catenin-alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, using Catenin-alpha1 Antibody. The lane on the right is blocked with the synthesized peptide.
