CTNNA1 Antibody

• Catalog Number: orb1257408
• Category: Antibodies
• Description: CTNNA1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 637-906 of human CTNNA1 (NP_001894.2).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 115kDa
• Target: CTNNA1
• UniProt ID: P35221
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: Catenin alpha-1, Alpha E-catenin, Cadherin-associa
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using CTNNA1 antibody (orb1257408) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat kidney using CTNNA1 antibody (orb1257408) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human vermiform appendix using CTNNA1 antibody (orb1257408) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using CTNNA1 antibody (orb1257408) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using CTNNA1 antibody (orb1257408) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200 ug extracts of 293T cells using 3 ug CTNNA1 antibody (orb1257408). Western blot was performed from the immunoprecipitate using CTNNA1 antibody (orb1257408) at a dilution of 1:500.