Catalase antibody

• Catalog Number: orb344573
• Category: Antibodies
• Description: Catalase antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Human, Mouse, Rat, Sheep
• Isotype: IgG
• Immunogen: Catalase [Bovine Liver]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000, IHC: User Optimized, WB: 1:10,000 - 1:50,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Catalase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Catalase [Bovine liver]. Cross reactivity against Catalase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated
• UniProt ID: P00432
• NCBI: AAI03067.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Catalase Antibody, Cas 1 antibody, CAT
• Note: For research use only
• Application notes: Anti-Catalase is tested for use in ELISA, western blot, and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Reduced samples of purified Catalase. Lane NR: Non-reduced samples of purified Catalase. Load: ~1ug of protein per lane using Catalase antibody