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CAT Rabbit Polyclonal Antibody

Catalog Number: orb100792

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb100792
CategoryAntibodies
DescriptionCAT Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, FC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityEquine, Mouse
ReactivityHuman, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from mouse Catalase
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000
Form/AppearanceLiquid
ConjugationUnconjugated
MW60 kDa
TargetCAT
UniProt IDP24270
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesCATA_HUMAN; Catalase; EC:1.11.1.6.
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NoteFor research use only
Expiration Date12 months from date of receipt.
CAT Rabbit Polyclonal Antibody

Blank control: HL60. Primary Antibody (green line): Rabbit Anti-Catalase antibody (orb100792), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

CAT Rabbit Polyclonal Antibody

Sample: Lane 1: Rat Kidney tissue lysates, Lane 2: Rat Placenta tissue lysates, Lane 3: Rat Heart tissue lysates, Lane 4: Human HeLa cell lysates, Lane 5: Human HepG2 cell lysates, Primary: Anti-CAT at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kDa, Observed band size: 60 kDa.

CAT Rabbit Polyclonal Antibody

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Catalase Polyclonal Antibody, Unconjugated (orb100792) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.