Catalase/CAT Antibody

• Catalog Number: orb19252
• Category: Antibodies
• Description: Goat polyclonal antibody to CAT
• Target: Catalase / CAT
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DKYNAEKPKNAIHT
• RRID: AB_10753324
• MW: 59.8
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 0.1-0.3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: Immunohistochemistry: In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration, 2-4ug/ml.WB: Approx 60kDa band observed in Human Kidney and Liver lysates (calculated MW of 59.8kDa according to NP_001743.1). Recommended concentration: 0.3-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti CAT antibody, anti catalase antibody, anti HG
• Research Area: Cardiovascular Disease, Epigenetics
• Note: For research use only
• Entrez: 847

References

Patche, Jessica et al. Diabetes-induced hepatic oxidative stress: a new pathogenic role for glycated albumin Free Radic Biol Med, 102, 133-148 (2017)

PubMed: 27890722

Reactivity

Mouse

Immunohistochemical staining of Human Spleen using CAT antibody

Western blot anlaysis of Human Kidney lysate using CAT antibody

Immunohistochemical staining of Human Liver using CAT antibody

Western blot analysis of Human Kidney (A) and Liver (B) and Mouse Kidney (C) and Liver (D) lysates using CAT antibody