Catalase/CAT Antibody

SKU: orb19252

Description

Goat polyclonal antibody to CAT

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 0.1-0.3 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: Immunohistochemistry: In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration, 2-4ug/ml.WB: Approx 60kDa band observed in Human Kidney and Liver lysates (calculated MW of 59.8kDa according to NP_001743.1). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Catalase / CAT
• Molecular Weight: 59.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti CAT antibody, anti catalase antibody, anti HGNC:1516 antibody, anti MGC138422 antibody, anti MGC138424 antibody

Immunohistochemical staining of Human Spleen using CAT antibody

Western blot anlaysis of Human Kidney lysate using CAT antibody

Immunohistochemical staining of Human Liver using CAT antibody

Western blot analysis of Human Kidney (A) and Liver (B) and Mouse Kidney (C) and Liver (D) lysates using CAT antibody

Quick Database Links

Gene Symbol

Catalase / CAT

NCBI Gene ID

847

References

Patche, Jessica et al. Diabetes-induced hepatic oxidative stress: a new pathogenic role for glycated albumin Free Radic Biol Med, 102, 133-148 (2017)

PubMed: 27890722

Reactivity

Mouse