CAT Antibody

SKU: orb344572

Description

Catalase antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000, WB: 1:10,000 - 1:50,000
• Reactivity: Bovine, Human, Mouse, Rat, Sheep
• Application Notes:
  Anti-Catalase is tested for use in ELISA, western blot, and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Catalase Antibody was produced by repeated immunizations with bovine liver catalase protein.
• Target: CAT
• Purity: Anti-Catalase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Catalase [Bovine liver]. Cross reactivity against Catalase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store Catalase Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge antibody if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Catalase Antibody, Cas 1 antibody, CAT antibody, Cs1 antibody, MGC138422 antibody, MGC138424 antibody

Immunohistochemistry of Catalase antibody. Tissue: human liver. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: Catalase antibody 1:400. Secondary antibody: Peroxidase goat anti-rabbit at 1:10000 for 45 min at RT. Localization: nuclear and occasionally cytoplasmic. Staining: Liver parenchyma: portal triads affected by moderate lymphoplasmacytic inflammation with minimal proliferation of bile ductules. Not obvious interstitial fibrosis. Cytoplasma of hepatocytes, diffuse positivity for the antibody anti-catalase. Neg Ctr (far right) normal rabbit IgG with Human liver. pH6.2 40X

Western Blot of Rabbit anti-Catalase antibody. Lane R: Reduced samples of purified Catalase. Lane NR: Non-reduced samples of purified Catalase. Load: ~1 ug of protein per lane. Primary antibody: Catalase antibody at 1:1000 for overnight at 4°C. Secondary antibody: Dylight488™ rabbit secondary antibody at 1:10000 for 1.5 hrs at RT. Block: orb348637 overnight at 4°C. Predicted/Observed size: 59.9 kDa, 55 kDa for catalase. Other band(s): none.

Quick Database Links

Gene Symbol

CAT

UniProt

P00432

RefSeq:AAI03067.1