Catalase rabbit pAb

• Catalog Number: orb766760
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Catalase
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Fish, Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from the C-terminal region of human CAT. AA range:478-527
• Concentration: 1 mg/ml
• Dilution range: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 60
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-CAT antibody, anti-Catalase antibody
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from 1) HT29, 2) A549, 3) HELA cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour. (Red) GAPDH Monoclonal Antibody (2B8) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour.

Immunofluorescence analysis of human-liver tissue. 1, Catalase Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunofluorescence analysis of human-liver tissue. 1, Catalase Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunofluorescence analysis of human-kidney-cancer tissue. 1, Catalase Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.