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Catalase rabbit pAb

Catalog Number: orb766760

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb766760
CategoryAntibodies
DescriptionRabbit polyclonal antibody to Catalase
ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ReactivityFish, Human, Mouse, Rat
Concentration1 mg/ml
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
ImmunogenThe antiserum was produced against synthesized peptide derived from the C-terminal region of human CAT. AA range:478-527
MW60
Tested applicationsELISA, IF, IHC-P, WB
Dilution rangeIF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesAnti-CAT antibody, anti-Catalase antibody
NoteFor research use only
Catalase rabbit pAb

Western blot analysis of lysates from 1) HT29, 2) A549, 3) HELA cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour. (Red) GAPDH Monoclonal Antibody (2B8) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour.

Catalase rabbit pAb

Immunofluorescence analysis of human-liver tissue. 1, Catalase Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Catalase rabbit pAb

Immunofluorescence analysis of human-liver tissue. 1, Catalase Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Catalase rabbit pAb

Immunofluorescence analysis of human-kidney-cancer tissue. 1, Catalase Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

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    ELISA,  IHC-P,  WB

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    100 μl, 50 μl
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    FC,  IHC-P,  WB

    Porcine, Rat, Zebrafish

    Human, Mouse

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    Unconjugated

    100 μl, 50 μl
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    WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
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    ELISA,  ICC,  IF,  IHC-Fr,  WB

    Mouse, Porcine, Rat

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • Catalase Rabbit pAb [orb2714789]

    IHC-P,  WB

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    100 μl, 50 μl