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Catalog Number | orb1929280 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB15351 |
Tested applications | FC, WB |
Predicted Reactivity | Rat |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:2000, WB: 1:1000, WB: 1:1000, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 84341 Da |
Target | This GIT1-Y554 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 533-561 amino acids from the C-terminal region of human GIT1-Y554. |
UniProt ID | Q9Y2X7 |
NCBI | NP_054749.2, NP_001078923.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | ARF GTPase-activating protein GIT1, ARF GAP GIT1, Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
GIT1-Y554 Antibody (C-term) western blot analysis in Hela cell line lysates (35 ug/lane).This demonstrates the GIT1 antibody detected the GIT1 protein (arrow).
GIT1-Y554 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35 ug/lane).This demonstrates the GIT1 antibody detected the GIT1 protein (arrow).
GIT1-Y554 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes: Anti-Phospho-GIT1-pY554. ctrl at 1:2000 dilution. Lane 1: U-2OS whole cell lysate. Lane 2: HUVEC whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.