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GIT1 (Y554) Antibody (C-term)

Catalog Number: orb1929280

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1929280
CategoryAntibodies
DescriptionAffinity Purified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB15351
Tested applicationsFC, WB
Predicted ReactivityRat
ReactivityHuman, Mouse
IsotypeRabbit IgG
Antibody TypePrimary Antibody
Dilution rangeWB: 1:2000, WB: 1:1000, WB: 1:1000, FC: 1:10~50
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
ConjugationUnconjugated
MW84341 Da
TargetThis GIT1-Y554 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 533-561 amino acids from the C-terminal region of human GIT1-Y554.
UniProt IDQ9Y2X7
NCBINP_054749.2, NP_001078923.1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesARF GTPase-activating protein GIT1, ARF GAP GIT1,
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NoteFor research use only
Expiration Date12 months from date of receipt.
GIT1 (Y554) Antibody (C-term)

GIT1-Y554 Antibody (C-term) western blot analysis in Hela cell line lysates (35 ug/lane).This demonstrates the GIT1 antibody detected the GIT1 protein (arrow).

GIT1 (Y554) Antibody (C-term)

GIT1-Y554 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35 ug/lane).This demonstrates the GIT1 antibody detected the GIT1 protein (arrow).

GIT1 (Y554) Antibody (C-term)

GIT1-Y554 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

GIT1 (Y554) Antibody (C-term)

All lanes: Anti-Phospho-GIT1-pY554. ctrl at 1:2000 dilution. Lane 1: U-2OS whole cell lysate. Lane 2: HUVEC whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.