GIT1 (Y554) Antibody (C-term)

• Catalog Number: orb1929280
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This GIT1-Y554 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 533-561 amino acids from the C-terminal region of human GIT1-Y554.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9Y2X7
• MW: 84341 Da
• Tested applications: FC, WB
• Dilution range: WB: 1:2000, WB: 1:1000, WB: 1:1000, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB15351
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: ARF GTPase-activating protein GIT1, ARF GAP GIT1,
• Note: For research use only
• NCBI: NP_001078923.1, NP_054749.2

GIT1-Y554 Antibody (C-term) western blot analysis in Hela cell line lysates (35 ug/lane).This demonstrates the GIT1 antibody detected the GIT1 protein (arrow).

GIT1-Y554 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35 ug/lane).This demonstrates the GIT1 antibody detected the GIT1 protein (arrow).

GIT1-Y554 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-Phospho-GIT1-pY554. ctrl at 1:2000 dilution. Lane 1: U-2OS whole cell lysate. Lane 2: HUVEC whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.