Caspase-3 (Pro and Active) Antibody [CAS3836]

• Catalog Number: orb1252886
• Category: Antibodies
• Description: Caspase-3 (Pro and Active) Antibody [CAS3836]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CAS3836
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1, kappa
• Immunogen: The large subunit domain of human Caspase-3 was used as the immunogen for this monoclonal antibody.
• Antibody Type: Primary Antibody
• Concentration: 0.5 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CASP3
• UniProt ID: P42574
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Caspase-3 Antibody: CPP32, SCA-1, CPP32B, CPP32, C
• Note: For research use only
• Application notes: Western blot: 2-4 ug/mlThis antibody will detect the pro form and p17 and p12 active forms of Caspase-3.
• Expiration Date: 12 months from date of receipt.

Western blot testing of staurosporine-treated Jurkat cells (2 hr, 2 uM) with Caspase-3 antibody at 2 ug/ml. The pro form is seen at ~32kD and active caspase-3 seen at ~17kD and ~12kD.