Caspase-3 Rabbit Polyclonal Antibody

• Catalog Number: orb10237
• Category: Antibodies
• Description: Caspase-3 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human caspase-3 p17 subunit (80-175/277aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 17/32 kDa
• Target: CASP3
• UniProt ID: P42574
• RRID: AB_10752011
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Caspase-3 subunit p17; cleaved Caspase 3; cleaved
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

M Olszewska, A Malcher, T Stokowy, N Pollock, AJ Berman, S Budkiewicz, M Kamieniczna Effects of Tcte1 knockout on energy chain transportation and spermatogenesis: implications for male infertility Human Reproduction Open, (2024)

PubMed:

Applications

IF

Reactivity

Mouse

Babaeenezhad, Esmaeel et al. D-Limonene Alleviates Acute Kidney Injury Following Gentamicin Administration in Rats: Role of NF-κB Pathway, Mitochondrial Apoptosis, Oxidative Stress, and PCNA Oxid Med Cell Longev, 2021, 6670007 (2021)

PubMed: 33510839

Caro-Astorga, Joaquín et al. Nascent histamine induces α-synuclein and caspase-3 on human cells Biochem Biophys Res Commun, 451, 580-586 (2014)

PubMed: 25124665

Oskoueian, Ehsan et al. Cytoprotective effect of palm kernel cake phenolics against aflatoxin B1-induced cell damage and its underlying mechanism of action BMC Complement Altern Med, 15, 392 (2015)

PubMed: 26518905

Oskoueian, Ehsan et al. Phorbol esters isolated from Jatropha meal induced apoptosis-mediated inhibition in proliferation of chang and Vero cell lines Int J Mol Sci, 13, 13816-13829 (2012)

PubMed: 23203036

Oskoueian, Ehsan et al. Phorbol esters from Jatropha meal triggered apoptosis, activated PKC-δ, caspase-3 proteins and down-regulated the proto-oncogenes in MCF-7 and HeLa cancer cell lines Molecules, 17, 10816-10830 (2012)

PubMed: 22964499

Sample: Lane 1: Raji (Human) Cell Lysate at 30 ug, Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 3: Lung (Mouse) Lysate at 40 ug, Lane 4: Lung (Rat) Lysate at 40 ug, Primary: Anti-Caspase-3 (orb10237) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35 kD, Observed band size: 37 kD.

Sample: Lane 1: Spleen (Mouse) Lysate at 40 ug, Lane 2: Lung (Mouse) Lysate at 40 ug, Lane 3: Lymph node (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Mouse) Lysate at 40 ug, Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 6: Spleen (Rat) Lysate at 40 ug, Lane 7: Lung (Rat) Lysate at 40 ug, Lane 8: Lymph node (Rat) Lysate at 40 ug, Lane 9: Cerebrum (Rat) Lysate at 40 ug, Lane 10: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-Caspase-3 (orb10237) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35 kD, Observed band size: 37 kD.

Tissue/cell: NIH/3T3 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Caspase-3) polyclonal Antibody, Unconjugated (orb10237) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.