Caspase-3 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb2563024
• Category: Antibodies
• Description: Caspase-3 Recombinant Rabbit Monoclonal Antibody
• Target: CASP3
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: A synthesized peptide derived from human Caspase 3 (176-277/277aa)
• UniProt ID: P42574
• MW: 12/29/32 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: IHC-F=1:100-500, IHC-P=1:100-500, IF=1:100-500, WB=1:1000-2000
• Antibody Type: Primary Antibody
• Clone Number: 4D2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: cleaved Caspase 3; cleaved Caspase-3; APOPAIN; CAS
• Research Area: Apoptosis, Caspases, Cell Biology, Cell Cycle, Met
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug total protein per lane of various lysates probed with Caspase-3 monoclonal antibody, unconjugated (orb2563024) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

25 ug total protein per lane of various lysates probed with Caspase-3 monoclonal antibody, unconjugated (orb2563024) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (orb2563024) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (orb2563024) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (orb2563024) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (orb2563024) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (orb2563024) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (orb2563024) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (orb2563024) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (orb2563024) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.